Leveraging SMART® technology and employing 5' RACE-like approach, SMARTer® Human TCR a/b Profiling Kit captures complete V(D)J variable regions of TCR transcripts, enabling users to analyze T-cell receptor diversity from human RNA samples or directly from cells. Kit yields quality sequencing libraries from as little as 10 ng to 3 g of total RNA obtained from peripheral blood leukocytes, or from 50–10,000 purified T cells. It can be used to generate data for both alpha- and beta-chain diversity.